Inactivation of E. coli & Listeria on plastic kitchen cutting boards by electrolyzed oxidizing water.
	The bactericidal effects of electrolyzed oxidizing water on bacterial strains in hospital infections
	Effect of electrolyzed water on wound healing.
	Effect of electrolyzed oxidizing water on excised burn-wounds in rats
	Decomposition of ethylene, a flower-senescence hormone, with electrolyzed anode water.

LIVESTOCK AND HORTICULTURE

	Treatment of Escherichia coli inoculated alfalfa sprouts with electrolyzed oxidizing water
	Antimicrobial interventions to reduce Salmonella species on poultry
	Use of Alkali Water on Dairy Farms

ALKALINE IONIZED WATER

Lab Analysis of Ionized water from Jupiter water ionizer

FOR THE LATEST LAB ANALYSIS COMPARISON -- 2008, CLICK HERE.

Oxidation-Reduction Potential and pH:

Alkaline minerals present:

Bacterial Content:

Report of Analysis by Certified Laboratories:

In this test, a specially concocted “brew” containing over-limit quantities of every commonly detected dangerous chemical was passed through a Jupiter Ionizer. The results show that contaminants were reduced in most cases to levels undetectable by the performing labs.

Test Laboratories: Seoul Metropolitan Government Institute of Health and Environment, Korea; Mizutek, USA; Microbac Laboratories, USA; Brandywine Science Center.

(Note: Eliminated indicates that no modern device can detect it within its range)

Summary

For more O.R.P. results, see here: Antioxidant Water

For more lab results see here and here.

For fluoride test results see here.

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Fluid replacement promotes optimal physical performance
Adequate fluid replacement helps maintain hydration and, promotes the health, safety, and optimal physical performance of individuals participating in regular physical activity.
Med Sci Sports Exercise
1996 Jan;28(1):i-vii.
American College of Sports Medicine position stand. Exercise and fluid replacement.
Convertino VA, Armstrong LE, Coyle EF, Mack GW, Sawka MN, Senay LC Jr, Sherman WM.
It is the position of the American College of Sports Medicine that adequate fluid replacement helps maintain hydration and, therefore, promotes the health, safety, and optimal physical performance of individuals participating in regular physical activity. This position statement is based on a comprehensive review and interpretation of scientific literature concerning the influence of fluid replacement on exercise performance and the risk of thermal injury associated with dehydration and hyperthermia. Based on available evidence, the American College of Sports Medicine makes the following general recommendations on the amount and composition of fluid that should be ingested in preparation for, during, and after exercise or athletic competition: 1) It is recommended that individuals consume a nutritionally balanced diet and drink adequate fluids during the 24-hr period before an event, especially during the period that includes the meal prior to exercise, to promote proper hydration before exercise or competition. 2) It is recommended that individuals drink about 500 ml (about 17 ounces) of fluid about 2 h before exercise to promote adequate hydration and allow time for excretion of excess ingested water. 3) During exercise, athletes should start drinking early and at regular intervals in an attempt to consume fluids at a rate sufficient to replace all the water lost through sweating (i.e., body weight loss), or consume the maximal amount that can be tolerated. 4) It is recommended that ingested fluids be cooler than ambient temperature [between 15 degrees and 22 degrees C (59 degrees and 72 degrees F])] and flavored to enhance palatability and promote fluid replacement. Fluids should be readily available and served in containers that allow adequate volumes to be ingested with ease and with minimal interruption of exercise. 5) Addition of proper amounts of carbohydrates and/or electrolytes to a fluid replacement solution is recommended for exercise events of duration greater than 1 h since it does not significantly impair water delivery to the body and may enhance performance. During exercise lasting less than 1 h, there is little evidence of physiological or physical performance differences between consuming a carbohydrate-electrolyte drink and plain water. 6) During intense exercise lasting longer than 1 h, it is recommended that carbohydrates be ingested at a rate of 30-60 g.h(-1) to maintain oxidation of carbohydrates and delay fatigue. This rate of carbohydrate intake can be achieved without compromising fluid delivery by drinking 600-1200 ml.h(-1) of solutions containing 4%-8% carbohydrates (g.100 ml(-1)). The carbohydrates can be sugars (glucose or sucrose) or starch (e.g., maltodextrin). 7) Inclusion of sodium (0.5-0.7 g.1(-1) of water) in the rehydration solution ingested during exercise lasting longer than 1 h is recommended since it may be advantageous in enhancing palatability, promoting fluid retention, and possibly preventing hyponatremia in certain individuals who drink excessive quantities of fluid. There is little physiological basis for the presence of sodium in n oral rehydration solution for enhancing intestinal water absorption as long as sodium is sufficiently available from the previous meal.

Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage.
Biochem Biophys Res Commun.
1997 May 8;234(1):269-74.

Shirahata S, Kabayama S, Nakano M, Miura T, Kusumoto K, Gotoh M, Hayashi H, Otsubo K, Morisawa S, Katakura Y.
Institute of Cellular Regulation Technology, Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan. sirahata@grt.kyushu-u.ac.jp
Active oxygen species or free radicals are considered to cause extensive oxidative damage to biological macromolecules, which brings about a variety of diseases as well as aging. The ideal scavenger for active oxygen should be 'active hydrogen'. 'Active hydrogen' can be produced in reduced water near the cathode during electrolysis of water. Reduced water exhibits high pH, low dissolved oxygen (DO), extremely high dissolved molecular hydrogen (DH), and extremely negative redox potential (RP) values. Strongly electrolyzed-reduced water, as well as ascorbic acid, (+)-catechin and tannic acid, completely scavenged O.-2 produced by the hypoxanthine-xanthine oxidase (HX-XOD) system in sodium phosphate buffer (pH 7.0). The superoxide dismutase (SOD)-like activity of reduced water is stable at 4 degrees C for over a month and was not lost even after neutralization, repeated freezing and melting, deflation with sonication, vigorous mixing, boiling, repeated filtration, or closed autoclaving, but was lost by opened autoclaving or by closed autoclaving in the presence of tungsten trioxide which efficiently adsorbs active atomic hydrogen. Water bubbled with hydrogen gas exhibited low DO, extremely high DH and extremely low RP values, as does reduced water, but it has no SOD-like activity. These results suggest that the SOD-like activity of reduced water is not due to the dissolved molecular hydrogen but due to the dissolved atomic hydrogen (active hydrogen). Although SOD accumulated H2O2 when added to the HX-XOD system, reduced water decreased the amount of H2O2 produced by XOD. Reduced water, as well as catalase and ascorbic acid, could directly scavenge H2O2. Reduced water suppresses single-strand breakage of DNA b active oxygen species produced by the Cu(II)-catalyzed oxidation of ascorbic acid in a dose-dependent manner, suggesting that reduced water can scavenge not only O2.- and H2O2, but also 1O2 and .OH.
PMID: 9169001 [PubMed - indexed for MEDLINE]

The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis.
Biophys Chem. 2004
Jan 1;107(1):71-82.
Hanaoka K, Sun D, Lawrence R, Kamitani Y, Fernandes G.
Bio-REDOX Laboratory Inc. 1187-4, Oaza-Ueda, Ueda-shi, Nagano-ken 386-0001, Japan. hanak@rapid.ocn.ne.jp
We reported that reduced water produced by electrolysis enhanced the antioxidant effects of proton donors such as ascorbic acid (AsA) in a previous paper. We also demonstrated that reduced water produced by electrolysis of 2 mM NaCl solutions did not show antioxidant effects by itself. We reasoned that the enhancement of antioxidant effects may be due to the increase of the ionic product of water as solvent. The ionic product of water (pKw) was estimated by measurements of pH and by a neutralization titration method. As an indicator of oxidative damage, Reactive Oxygen Species- (ROS) mediated DNA strand breaks were measured by the conversion of supercoiled phiX-174 RF I double-strand DNA to open and linear forms. Reduced water had a tendency to suppress single-strand breakage of DNA induced by reactive oxygen species produced by H2O2/Cu (II) and HQ/Cu (II) systems. The enhancement of superoxide anion radical dismutation activity can be explained by changes in the ionic product of water in the reduced water.
PMID: 14871602 [PubMed - in process]

Oxygen Radical Absorbance Capacity

High-ORAC Foods May Slow Aging
Agricultural Research Service, USDA, February 8, 1999
Foods that score high in an antioxidant analysis called ORAC may protect cells and their components from oxidative damage, according to studies of animals and human blood at the Agricultural Research Services Human Nutrition Research Center on Aging at Tufts in Boston. ARS is the chief scientific agency of the U.S. Department of Agriculture.
ORAC, short for oxygen radical absorbance capacity, is a test tube analysis that measures the total antioxidant power of foods and other chemical substances. Early findings suggest that eating plenty of high-ORAC fruits and vegetables, such as spinach and blueberries, may help slow the processes associated with aging in both body and brain. If these findings are borne out in further research, young and middle-aged people may be able to reduce risk of diseases of aging (including senility) simply by adding high-ORAC foods to their diets, said ARS Administrator Floyd P. Horn.
In the studies,eating plenty of high-ORAC foods:

• Raised the antioxidant power of human blood 10 to 25%

• Prevented some loss of long-term memory and learning ability in middle-aged rats

• Maintained the ability of brain cells in middle-aged rats to respond to a chemical stimulus-a function that normally decreases with age.

• Protected rats' tiny blood vessels (capillaries) against oxygen damage.

Alkaline Water protection against the toxic effects of mercury

Hair element concentrations in females in one acid and one alkaline area in southern Sweden, Rosborg I, Nihlgard B, Gerhardsson L.

Department of Occupational and Environmental Medicine, Lund University, Sweden. ingegerd.rosborg@ymed.lu.se

Concentrations of 34 trace elements in hair have been determined in 47 females from an acid region in southern Sweden, who were compared with 43 females from an alkaline area. The concentrations of these elements in hair and drinking water were determined by inductively coupled plasma optical emission spectroscopy and inductively coupled plasma mass spectrometry. The hair concentrations of boron and barium were significantly higher (p < 0.001) in hair samples from the acid region, the hair levels of calcium, strontium, molybdenum, iron, and selenium were significantly higher (p < 0.001) in the alkaline region. For some metals, e.g. calcium, lead, molybdenum, and strontium, there were positive correlations between the concentrations in hair and water (rs = 0.34-0.57; p < or = 0.001), indicating the importance of intake from minerals in water. The increased ratio of selenium/mercury concentrations in hair samples obtained in the alkaline district (p < 0.001) indicates that these subjects may have better protection against the toxic effects of mercury.

PMID: 14703901 [PubMed - indexed for MEDLINE]

Use of Ionized water in hypochlorhydria or achlorhydria
Prof. Kuninaka Hironage, Head of Kuninaka Hospital
"Too many fats in the diets, which lead to the deposition of cholesterol on the blood vessels, which in turn constrict the blood flow, cause most illnesses such as high blood pressure. In accordance with the theory of Professor Gato of Kyushu University on Vitamin K (because vitamin K enables the blood calcium to increase ), or the consumption of more antioxidant water, the effectiveness of the increase in the calcium in high blood pressure is most significant. The consumption of alkaline antioxidant water for a period of 2 to 3 months, I have observed the blood pressure slowly drop, due to the water's solvent ability, which dissolves the cholesterol in the blood vessels."

Use of Ionized water for gynecological conditions
Prof. Watanabe Ifao, Watanabe Hospital
"Ionized alklaine antioxidant water improves body constituents and ensures effective healing to many illnesses. The uses of antioxidant water in gynecological patients have proved to be very effective. The main reason for its effectiveness is that this water can neutralize toxins.
When given antioxidant water to pre-eclamptic toxemia cases, the results are most significant. During my long years of servicing the pre-eclamptic toxemia cases, I found that the women with pre-eclamptic toxemia who consumed antioxidant water tend to deliver healthier babies with stronger muscles. A survey report carried out on babies in this group showed intelligence above average."

CLINICAL Impovements Obtained From The Intake Of Reduced Water

Extracts from " Presentation At The Eight Annual International Symposium On man And His Environment in Health And Disease" on February 24th 1990, at The Grand Kempinski Hotel, Dalls, Texas, USA by Dr. H. Hayashi, M.D. and Dr. M Kawamura, M.D., on : -

(THE CONCEPT OF PREHEPATIC MEDICINES)

Since the introduction of alkaline ionic water in our clinic in 1985, we have had the following interesting clinical experiences in the use of this type of water. By the use of alkaline ionic water for drinking and the preparation of meals for our in-patients, we have noticed :-
Declines in blood sugar levels in diabetic patients.
Improvements in peripheral circulation in diabetic gangrene.
Declines in uric acid levels in patients with gout.
Improvements in liver function exams in hepatic disorders.
Improvements in gastroduodenal ulcers and prevention of their recurrences.
Improvements in hypertension and hypotension.
Improvements in allergic disorders such as asthma, urticaria, rhinites and atopic dermatitis.
Improvements in persistent diarrhoea which occurred after gastrectomy.
Quicker improvements in post operative bower paralysis.
Improvements in serum bilirubin levels in new born babies.
Being confirming clinical improvements, we have always observed changes of stools of the patients, with the colour of their feces changing from black-brown colour to a brighter yellow-brown one, and the odour of their feces becoming almost negligible.
The number of patients complaining of constipation also decreased markedly. The change of stool findings strongly suggests that alkaline ionic water intake can decrease the production of putrefied or pathogenic metabolites.
Devices to produce reduced water were introduced into our clinic in May 1985. Based on the clinical experiences obtained in the past 15 years, it can be said that introduction of electrolyzed-reduced water for drinking and cooking purpose for in-patients should be the very prerequisite in our daily medical practices. Any dietary recipe cannot be a scientific one if property of water is not taken by the patients is not taken into consideration.
The Ministry of Health and Welfare in Japan announced in 1965 that the intake of reduced water is effective for restoration of intestinal flora metabolism.

Toxin Neutralisation
Prof. Kuwata Keijiroo, Doctor of Medicine
"In my opinion, the wonder of antioxidant water is the ability to neutralize toxins; but it is not a medicine. The difference is that medicine can only apply to individual cases, whereas the antioxidant water can be consumed generally and its neutralizing power is something which is very much unexpected. Now, in brief, let me introduce to you a heart disease case and how it was cured.
The patient was a 35 years old male suffering from vascular heart disease. For 5 years, his sickness deteriorated. He was in the Setagays Government Hospital for treatment.

During those 5 years, he had been in and out of the hospital 5 to 6 times. He had undergone high tech examinations such as angiogram by injecting VINYL via the vein into the heart. He consulted and sought treatment from many good doctors where later he underwent a major surgical operation. Upon his discharge from the hospital, he quit his job to convalesce. However, each time when his illness relapsed, the attack seemed to be even more severe.
Last year, in August, his relatives were in despair and expected he would not live much longer. It so happened at that time that the victim's relative came across antioxidant water processor. His illness responded well and he is now on the road to recovery."

(In the United States, cardiovascular diseases account for more than one-half of the approximate 2 million deaths occurring each year…. It is estimated that optimal conditioning of drinking water could reduce this cardiovascular disease mortality rate by as much as 15 percent in the United States) From: Report of the Safe Drinking Water Committee of the National Academy of Sciences, 1977

Eczema
Prof. Tamura Tatsuji, Keifuku Rehabilitation Center
"Eczema is used to describe several varieties of skin conditions, which have a number of common features. The exact causes of eczema are not fully understood. I many cases, eczema can be attributed to external irritants. Let me introduce a patient who recovered from skin disease after consuming the antioxidant water. This patient suffered 10 years of eczema and could not be cured effectively even under specialist treatment. This patient, who is 70 years of age, is the president of a vehicle parts company. After the war, his lower limbs suffered acute eczema, which later became chronic. He was repeatedly treated in a specialist skin hospital.

The left limb responded well to treatment, but not so on the right limb. He suffered severe itchiness, which, when scratched led to bleeding. During the last 10 years, he was seen and treated by many doctors. When I first examined him, his lower limb around the joints was covered with vesicles. Weeping occurred owing to serum exuding from the vesicles.

I advised him to try consuming antioxidant water. He bought a unit and consumed the antioxidant water religiously and used the acidic water to bathe the affected areas. After 2 weeks of treatment the vesicles dried up. The eczema was completely cleared without any relapse after 1½ month."

Antibacterial effect of electrolyzed water on oral bacteria
by SH Lee and BK Choi

"Electrolyzed tap water was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. In addition, the water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. Ionized water significantly reduced the growth of all periodontopathogens in culture and on toothbrushes, and that of aerobic and anaerobic bacteria in saliva, when compared to the effect of tap water."

Allergies

Prof. Kuninaka Hironaga, Head of Kuninaka Hospital
"Mr. Yamada, the head of Police Research Institute, suffered from severe allergy. He was treated repeatedly by skin specialist, but with no success. Then he started consuming antioxidant water. The allergy responded very well and was soon completely cured. No relapse had occurred, although he had taken all kinds of food. He was most grateful and excited about this treatment.

As for myself, I had also suffered severe allergy. Ever since I began to consume antioxidant water, the allergy has recovered. Since then, I started a research on the effectiveness of antioxidant water.

I discovered that most allergies are due to acidification of body condition and is also related to consuming too much meat and sugar. In every allergy case, the patient's antioxidant minerals are excessively low which in turn lower the body resistance significantly. The body becomes overly sensitive and develops allergy easily. To stabilize the sensitivity, calcium solution in injected into the vein. Therefore, it is clear that the antioxidant water has ionic calcium, which can help alleviate allergy.

The ionic calcium not only enhances the heart, urination, and neutralization of toxins but controls acidity. It also enhances the digestive system and liver function. This will promote natural healing power and hence increase its resistance to allergy. In some special cases of illness, which do not respond to drugs, it is found, it is found to respond well to antioxidant water."

Digestive Problems
Prof. Kogure Keizou, Kogure Clinic of Juntendo Hospital

"The stomach is readily upset both by diseases affecting the stomach and by other general illnesses. In addition, any nervous tension or anxiety frequently causes gastric upset.

The important role of antioxidant water in our stomach is to neutralize the secretion and strengthen its functions. Usually, after consuming the antioxidant water for 1 to 3 minutes, the gastric juice increase to 1½ times. For those suffering from achlorhydria ( low in gastric juice ) the presence of antioxidant water will stimulate the stomach cells to secrete more gastric juice. This in turn enhances digestion and absorption of minerals. However, those with hyperchlorhydria ( high in gastric juice ), the antioxidant water neutralizes the excessive gastric juice. Hence, it does not create any adverse reaction. According to the medical lecturer from Maeba University, the pH of the gastric secretion will still remain normal when antioxidant water is consumed. This proves the ability of the antioxidant water to neutralize as well as to stimulate the secretion."

Diabetes
TWO ABSTRACTS and ONE REPORT ON DIABETES / ALKALINE WATER RESEARCH

Effects of Alkaline Ionized Water on Spontaneously diabetic GK-rats fed Sucrose

Jin Man Kim Division of Life Science, R&D center, Sunkyong Industries, Kazuhito Yokoyama Department of Public Health, Faculty of Medicine, The University of Tokyo

This study was carried out to evaluate the effects of alkaline ionized water (AIW) on spontaneously diabetic GK-rats fed sucrose for aggravation of diabetes mellitus.

One half of the 32 GK rats was given AIW and the other was given tap water (TW). These two groups were further divided into two subgroups by fed with or without 30% sucrose solution (8 in each group). In blood glucose level, sucrose fed TW group was significantly higher than the other groups. Sucrose fed both AIW and TW groups were significantly increased in body weight as compared to TW group. In serum malondialdehyde (MDA), a marker of lipid peroxide, sucrose fed TW group was significantly higher than AIW and TW groups.

It is suggested that AIW (Alkaline Ionized Water) supplementation may inhibit the increase of blood glucose and lipid peroxide levels in diabetes mellitus.

Protective mechanism of reduced water against alloxan-induced pancreatic ?-cell damage: Scavenging effect against reactive oxygen species

Cytotechnology 40: 139–149, 2002. Netherlands.139

Yuping Li1, Tomohiro Nishimura1, Kiichiro Teruya1, et al , Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan; 2 Nihon Trim Co. Ltd., 1-8-34 Oyodonaka, Kita-ku, Osaka, Japan: 3 Hita TenryosuiCo. Ltd., 647 Nakanoshima, Hita, Oita, Japan; 4 Center for Holistic Medicine and Naturopathy, Schmallenberg-Nordenau, Germany Author for correspondence; E-mail: sirahata@grt.kyushu-u.ac.jp

Abstract

Reactive oxygen species (ROS) cause irreversible damage to biological macromolecules, resulting in many diseases.Reduced water (RW) such as hydrogen-rich electrolyzed reduced water and natural reduced waters like Hita Tenryosui water in Japan and Nordenau water in Germany that are known to improve various diseases, could protect a hamster pancreatic ? cell line, HIT-T15 from alloxan-induced cell damage. Alloxan, a diabetogenic compound, is used to induce type 1 diabetes mellitus in animals. Its diabetogenic effect is exerted via the production of ROS. Alloxan-treated HIT-T15 cells exhibited lowered viability, increased intracellular ROS levels, elevated cytosolic free Ca2+ concentration, DNA fragmentation, decreased intracellular ATP levels and lowering of glucose-stimulated release of insulin. RWcompletely prevented the generation of alloxan-inducedROS, increase of cytosolic Ca2+ concentration, decrease of intracellular ATP level, and lowering of glucose-stimulated insulin release, and strongly blocked DNA fragmentation, partially suppressing the lowering of viability of alloxan-treated cells. Intracellular ATP levels and glucose-stimulated insulin secretion were increased by RW to 2–3.5 times and 2–4 times, respectively, suggesting that RW enhances the glucose-sensitivity and glucose response of ?-cells. The protective activity of RWwas stable at 4 ?C for over a month, but was lost by autoclaving. These results suggest that RW protects pancreatic ?-cells from alloxan-induced cell damage by preventing alloxan-derived ROS generation. RW may be useful in preventing alloxan-induced type 1-diabetes mellitus.

Diabetes

Prof. Kuwata Keijiroo, Doctor of Medicine

"When I was serving in the Fire Insurance Association, I used to examine many diabetic patients. Besides treating them with drugs, I provided them with antioxidant water. After drinking antioxidant water for one month, 15 diabetic patients were selected and sent to Tokyo University for further test and observations.
Initially, the more serious patients were a bit apprehensive about the treatment. When the antioxidant water was consumed for some time, the sugar in the blood and urine ranged from a ratio of 300 mg/l to 2 mg / dc. There was a time where the patient had undergone 5 to 6 blood tests a day and detected to be within normal range. Results also showed that even 1 ½ hour after meals, the blood sugar and urine ratio was 100 mg/dc: 0 mg/dc . The sugar in the urine has completely disappeared."

NOTE: More Americans than ever before are suffering from diabetes, with the number of new cases averaging almost 800,000 each year. The disease has steadily increased in the United States since 1980, and in 1998, 16 million Americans were diagnosed with diabetes (10.3 million diagnosed; 5.4 million undiagnosed). Diabetes is the seventh leading cause of death in the United States, and more than 193,000 died from the disease and its related complications in 1996. From: U. S. Department of Health and Human Services, October 13, 2000 Fact Sheet.

Use of Ionized water in treating Acidosis

Prof. Hatori Tasutaroo, Head of Akajiuiji Blood Centre, Yokohama Hospital, Faitama District
"Due to a higher standard of living, our eating habits have changed. We consume too much proteins, fats and sugar. The excess fats and carbohydrates are in the body as fats. In the present lifestyles, Americans are more extravagant on food compared to the Japanese. Due to this excessive intake obesity is a significant problem. Normally, one out of five males and one out of four females is obese.

The degree of "burn-out" in food intake largely depends on the amount on intake of vitamins and minerals. When excessive intake of proteins, carbohydrates and fats occurs, the requirement for vitamins and minerals increases. However, there is not much research carried out pertaining to the importance of vitamins and minerals.

Nowadays, many people suffer from acidification that leads to diabetes, heart diseases, cancer, live and kidney diseases. If our food intake can be completely burned off, then there is no deposition of fats. Obviously, there will be no acidification problem and hence there should not be any sign of obesity.

The antioxidant water contains an abundance of ionic calcium. This ionic calcium helps in the "burn-off" process. By drinking antioxidant water, it provides sufficient minerals for our body. As a result, we do not need to watch our diet to stay slim.

Hence, antioxidant water is a savior for those suffering from obesity and many adult diseases, providing good assistance in enhancing good health."

REDUCED WATER FOR PREVENTION OF DISEASES

Dr.Sanetaka Shirahata
Graduate school of Genetic Resources Technology, Kyushu University,
6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
It has long been established that reactive oxygen species (ROS) cause many types
of damage to biomolecules and cellular structures, that, in turn result in the development of a variety of pathologic states such as diabetes, cancer and aging. Reduced water is defined as anti-oxidative water produced by reduction of water. Electrolyzed reduced water (ERW) has been demonstrated to be hydrogen-rich water and can scavenge ROS in vitro (Shirahata et al., 1997). The reduction of proton in water to active hydrogen (atomic hydrogen, hydrogen radical) that can scavenge ROS is very easily caused by a weak current, compared to oxidation of hydroxyl ion to oxygen molecule. Activation of water by magnetic field, collision, minerals etc. will also produce reduced water containing active hydrogen and/or hydrogen molecule. Several natural waters such as Hita Tenryosui water drawn from deep underground in Hita city in Japan, Nordenau water in Germany and Tlacote water in Mexico are known to alleviate various diseases. We have developed a sensitive method by which we can detect active hydrogen existing in reduced water, and have demonstrated that not only ERW but also natural reduced waters described above contain active hydrogen and scavenge ROS in cultured cells. ROS is known to cause reduction of glucose uptake by inhibiting the insulin-signaling pathway in cultured cells. Reduced water scavenged intracellular ROS and stimulated glucose uptake in the presence or absence of insulin in both rat L6 skeletal muscle cells and mouse 3T3/L1 adipocytes. This insulin-like activity of reduced water was inhibited by wortmannin that is specific inhibitor of PI-3 kinase, a key molecule in insulin signaling pathways. Reduced water protected insulin-responsive cells from sugar toxicity and improved the damaged sugar tolerance of type 2 diabetes model mice, suggesting that reduced water may improve insulin-independent diabetes mellitus. Cancer cells are generally exposed to high oxidative stress. Reduced water cause impaired tumor phenotypes of human cancer cells, such as reduced growth rate, morphological changes, reduced colony formation ability in soft agar, passage number-dependent telomere shortening, reduced binding abilities of telomere binding proteins and suppressed metastasis. Reduced water suppressed the growth of cancer cells transplanted into mice, demonstrating their anti-cancer effects in vivo. Reduced water will be applicable to not only medicine but also food industries, agriculture, and manufacturing industries.

Shirahata, S. et al.: Electrolyzed reduced water scavenges active oxygen species and protects DNA from oxidative damage. Biochem. Biophys. Res. Commun., 234, 269174, 1997.

Clinical evaluation of alkaline ionized water for abdominal complaints: Placebo controlled double blind tests
by Hirokazu Tashiro, Tetsuji Hokudo, Hiromi Ono, Yoshihide Fujiyama, Tadao Baba (National Ohkura Hospital, Dept. of Gastroenterology; Institute of Clinical Research, Shiga University of Medical Science, Second Dept. of Internal Medicine)
Effect of alkaline ionized water on abdominal complaints was evaluated by placebo controlled double blind tests. Overall scores of improvement using alkaline ionized water marked higher than those of placebo controlled group, and its effect proved to be significantly higher especially in slight symptoms of chronic diarrhoea and abdominal complaints in cases of general malaise. Alkaline ionized water group did not get interrupted in the course of the test, nor did it show serious side effects nor abnormal test data. It was confirmed that alkaline ionized water is safer and more effective than placebos.

Summary
Effect of alkaline ionized water on abdominal complaints was clinically examined by double blind tests using clean water as placebo. Overall improvement rate was higher for alkaline ionized water group than placebo group and the former proved to be significantly more effective than the other especially in cases of slight symptoms. Examining improvement rate for each case of chronic diarrhoea, constipation and abdominal complaints, alkaline ionized water group turned out to be more effective than placebo group for chronic diarrhoea, and abdominal complaints. The test was stopped in one case of chronic diarrhoea, among placebo group due to exacerbation, whereas alkaline ionized water group did not stop testing without serious side effects or abnormal test data in all cases. It was confirmed that alkaline ionized water is more effective than clean water against chronic diarrhoea, abdominal complaints and overall improvement rate (relief of abdominal complaints) and safer than clean water.
Introduction
Since the approval of alkaline ionized water electrolyzers by Pharmaceutical Affairs Law in 1966 for its antacid effect and efficacy against gastrointestinal disorders including hyperchylia, indigestion, abnormal gastrointestinal fermentation and chronic diarrhoea, they have been extensively used among patients. However, medical and scientific evaluation of their validity is not established. In our study, we examined clinical effect of alkaline ionized water on gastrointestinal disorders across many symptoms in various facilities. Particularly, we studied safety and usefulness of alkaline ionized water by doubleblind tests using clean water as a control group.

Test subjects and methods

163 patients (34 men, 129 women, age 21 to 72, average 38.6 years old) of indigestion, abnormal gastrointestinal fermentation (with abnormal gas emission and rugitus) and abdominal complaints caused by irregular dejection (chronic diarrhoea, or constipation) were tested as subjects with good informed consent. Placebo controlled double blind tests were conducted using alkaline ionized water and clean water at multiple facilities. An alkaline ionized water electrolyzer sold commercially was installed with a pump driven calcium dispenser in each of the subject homes. Tested alkaline ionized water had pH at 9.5 and calcium concentration at 30ppm. Each subject in placebo group used a water purifier that has the same appearance as the electrolyzer and produces clean water.

The tested equipment was randomly assigned by a controller who scaled off the key code which was stored safely until the tests were completed and the seal was opened again.

Water samples were given to each patient in the amount of 200ml in the morning with the total of 50OmI or more per day for a month. Before and after the tests, blood, urine and stool were tested and a log was kept on the subjective symptoms, bowel movements and accessory symptoms. After the tests, the results were analyzed based on the log and the test data.

Test Results

1. Symptom
Among 163 tested subjects, alkaline ionized water group included 84 and placebo group 79. Background factors such as gender, age and basal disorders did not contribute to significant difference in the results.

2. Overall improvement rate

As to overall improvement rate of abdominal complaints, alkaline ionized water group had 2 cases of outstanding improvement (2.5%), 26 cases of fair improvement (32.1%), 36 cases of slight improvement (44.4%), 13 cases of no change (16%) and 4 cases of exacerbation (4.9%), whereas placebo group exhibited 4 (5.2%), 19 (24.7%), 27 (35.1%), 25 (32.5%) and 2 cases (2.6%) for the same category. Comparison between alkaline ionized water and placebo groups did not reveal any significant difference at the level of 5% significance according to the Wilcoxon test, although alkaline ionized water group turned out to be significantly more effective than placebo group at the level of p value of 0.22.

Examining overall improvement rates by a 7, 2 test (with no adjustment for continuity) between the effective and noneffective groups, alkaline ionized water group had 64 (79%) of effective cases and 17 cases (21%) of non effective cases, whereas placebo group had 50 (64.9%) and 27 (35.1%) cases respectively. The result indicated that alkaline ionized water group was significantly more effective than placebo group at the level of p value of 0.0.48.

Looking only at 83 slight cases of abdominal complaints, overall improvement rate for alkaline ionized water group

(45 cases) was composed of 11 cases (242%) of fair improvement, 22 cases (48.9%) of slight improvement, 17 cases (44.7%) of no change and 3 cases (6.7%) of exacerbation, whereas placebo group (38 cases) had 3 (7.8%), 17 (44.7%), 17 (44.7%) and 1 (2.6%) cases for the same category. Alkaline ionized water group was significantly more effective than placebo group according to the comparison between the groups (p value = 0.033).

3. Improvement rate by basal symptom

Basal symptoms were divided into chronic diarrhea, constipation and abdominal complaints (dyspepsia) and overall improvement rate was evaluated for each of them to study effect of alkaline ionized water. In case of chronic diarrhoea, alkaline ionized water group resulted in 94.1% of effective cases and 5.9% of non effective cases. Placebo group came up with 64,7% effective and 35.3% non effective. These results indicate alkaline ionized water group proved to be significantly more effective than placebo group. In case of slighter chronic diarrhoea, comparison between groups revealed that alkaline ionized water group is significantly more effective than placebo group (p=0.015). In case of constipation, alkaline ionized water group consisted of 80.5% of effective and 19.5% of non effective cases, whereas placebo group resulted in 73.3% effective and 26.3 non effective. As to abdominal complaints (dyspepsia), alkaline ionized water group had 85.7% of effective and 14.3% non effective cases while placebo group showed 47.1% and 62.9% respectively. Alkaline ionized water group proved to be significantly more effective than placebo group (p=0.025).

4. Safety

Since one case of chronic diarrhoea, in placebo group saw exacerbation, the test was stopped. There was no such cases in alkaline ionized water group. Fourteen cases of accessory symptoms, 8 in alkaline ionized water group and 6 in placebo group, were observed, none of which were serious. 31 out of 163 cases (16 in alkaline ionized water group, 15 in placebo group) exhibited fluctuation in test data, although alkaline ionized water group did not have any problematic fluctuations compared to placebo group. Two cases in placebo group and one case in alkaline ionized water group have seen K value of serum climb up and resume to normal value after re testing which indicates the value changes were temporary.

Conclusion

As a result of double blind clinical tests of alkaline ionized water and clean water, alkaline ionized water was proved to be more effective than clean water against chronic diarrhoea, abdominal complaints (dyspepsia) and overall improvement rate (relief from abdominal complaints). Also, safety of alkaline ionized water was confirmed which clinically verifies its usefulness.

Selective stimulation of the growth of anaerobic microflora in the human intestinal tract by electrolyzed reducing water

Vorobjeva NV, Med Hypotheses. 2005;64(3):543-6.

96-99% of the "friendly" or residential microflora of intestinal tract of humans consists of strict anaerobes and only 1-4% of aerobes. Many diseases of the intestine are due to a disturbance in the balance of the microorganisms inhabiting the gut. The treatment of such diseases involves the restoration of the quantity and/or balance of residential microflora in the intestinal tract. It is known that aerobes and anaerobes grow at different oxidation-reduction potentials (ORP). The former require positive E(h) values up to +400 mV. Anaerobes do not grow unless the E(h) value is negative between -300 and -400 mV. In this work, it is suggested that prerequisite for the recovery and maintenance of obligatory anaerobic microflora in the intestinal tract is a negative ORP value of the intestinal milieu. Electrolyzed reducing water with E(h) values between 0 and -300 mV produced in electrolysis devices possesses this property. Drinking such water favours the growth of residential microflora in the gut. A sufficient array of data confirms this idea. However, most researchers explain the mechanism of its action by an antioxidant properties destined to detox the oxidants in the gut and other host tissues. Evidence is presented in favour of the hypothesis that the primary target for electrolyzed reducing water is the residential microflora in the gut.

Vormann J, Worlitschek M, Goedecke T, Silver B, Supplementation with alkaline minerals reduces symptoms of patients with chronic low back pain, J Trace Elem. Med. Biol. Vol. 15, pp. 179-183, 2001

Abstract: The cause of low back pain is heterogeneous, it has been hypothesized that a latent chronic acidosis might contribute to these symptoms. It was tested whether a supplementation with alkaline minerals would influence symptoms in patients with low back pain symptoms. In an open prospective study 82 patients with chronic low back pain received daily 30 g of a lactose based alkaline multimineral supplement (Basica) over a period of 4 weeks in addition to their usual medication. Pain symptoms were quantified with the "Arhus low back pain rating scale" (ARS). Mean ARS dropped highly significant by 49% from 41 to 21 points after 4 weeks supplementation. In 76 out of 82 patients a reduction in ARS was achieved by the supplementation. Total blood buffering capacity was significantly increased from 77.69 ± 6.79 to 80.16 ± 5.24 mmol/L (mean ± SEM, n=82, p < 0.001) and also blood pH rose from 7.456 ± 0.007 to 7.470 ± 0.007 (mean ± SEM, n=75, p < 0.05). Only intracellular magnesium increased by 11% while other intracellular minerals were not significantly changed in sublingual tissue as measured with the EXA-test. Plasma concentrations of potassium, calcium, iron, copper, and zinc were within the normal range and not significantly influenced by the supplementation. Plasma magnesium was slightly reduced after the supplementation (-3%, p < 0.05). The results show that a disturbed acid-base balance may contribute to the symptoms of low back pain. The simple and safe addition of an alkaline multimineral preparate was able to reduce the pain symptoms in these patients with chronic low back pain.

Physiological effects of alkaline ionized water: Effects on metabolites produced by intestinal fermentation
by Takashi Hayakawa, Chicko Tushiya, Hisanori Onoda, Hisayo Ohkouchi, Harul-~to Tsuge (Gifu University, Faculty of Engineering, Dept. of Food Science)

We have found that long-term ingestion of alkaline ionized water (AIW) reduces cecal fermentation in rats that were given highly fermentable commercial diet (MF: Oriental Yeast Co., Ltd.). In this experiment, rats were fed MF and test water (tap water, AIW with pH at 9 and 10) for about 3 months. Feces were collected on the 57th day, and the rats were dissected on the 88th day. The amount of ammonium in fresh feces and cecal contents as well as fecal free-glucose tended to drop down for the AIW group. In most cases, the amount of free-amino acids in cecal contents did not differ sign- icantly except for cysteine (decreased in AIW with pH at 10) and isoleucine (increased in AIW with pH at 10).

Purpose of tests
Alkaline ionized water electrolyzers have been approved for manufacturing in 1965 by the Ministry of Health and Welfare as medical equipment to produce medical substances. Alkaline ionized water (AIW) produced by this equipment is known to be effective against gastrointestinal fermentation, chronic diarrhea, indigestion and hyperchylia as well as for controlling gastric acid.*1 This is mainly based on efficacy of the official calcium hydroxide. *2 By giving AIW to rats for a comparatively long time under the condition of extremely high level of intestinal fermentation, we have demonstrated that AIW intake is effective for inhibition of intestinal fermentation when its level is high based on some test results where AIW worked against cecal hypertrophy and for reduction in the amount of short-chain fatty acid that is the main product of fermentation.*3 We have reported that this is caused by the synergy between calcium level generally contained in AIW (about 50ppm) and the value of pH, and that frequency of detecting some anaerobic bacteria tends to be higher in alkaline ionized water groups than the other, although the bacteria count in the intestine does not have significant difference. Based on these results, we made a judgment that effect of taking AIW supports part of inhibition mechanism against abnormal intestinal fermentation, which is one of the claims of efficacy that have been attributed to alkaline ionized water electrolyzers. *4 On the other hand, under the dietary condition of low intestinal fermentation, AIW uptake does not seem to inhibit fermentation that leads us to believe that effect of AIW uptake is characteristic of hyper-fermentation state. Metabolites produced by intestinal fermentation include indole and skatole in addition to organic acids such as short-chain fatty acid and lactic acid as well as toxic metabolites such as ammonium, phenol and pcresol. We do not know how AIW uptake would affect the production of these materials. In this experiment, we have tested on ammonium production as explained in the following sections.

Testing methods

Four-week-old male Wistar/ST Clean rats were purchased from Japan SLC Co., Ltd. and were divided into 3 groups of 8 each after preliminary breeding. AIW of pH 9 and 10 was produced by an electrolyzer Mineone ROYAL NDX3 1 OH by Omco Co., Ltd. This model produces AIW by electrolyzing water with calcium lactate added. On the last day of testing, the rats were dissected under Nembutal anesthesia to take blood from the heart by a heparin-treated syringe. As to their organs, the small intestines, cecum and colon plus rectum were taken out from each of them. The cecurn was weighed and cleaned with physiological saline after its contents were removed, and the tissue weight was measured after wiping out moisture. Part of cecal contents was measured its pH, and the rest was used to assay ammonium concentration. The amount of ammonium contained in fresh feces and cecal contents was measured by the Nessler method after collecting it in the extracted samples using Conway's micro-diffusion container. Fecal free-glucose was assayed by the oxygen method after extraction by hot water. Analysis of free amino acids contained in cecal contents was conducted by the Waters PicoTag amino acid analysis system.

Test results and analyses

No difference was found in the rats' weight gain, water and feed intake and feeding efficiency, nor was any particular distinction in appearance identified. The length of the small intestines and colon plus rectum tended to decline in AIW groups. PH value of cecal contents was higher and the amount of fecal free-glucose tended to be lower in AIW groups than the control group. Since there was no difference in fecal discharge itself, the amount of free-glucose discharged per day was at a low level. The amount of discharged free-glucose in feces is greater when intestinal fermentation is more intensive, which indicates that intestinal fermentation is more inhibited in AIW groups than the control group. Ammonium concentration in cecal contents tends to drop down in AIW groups (Fig. 1). This trend was most distinctive in case of fresh feces of one of AIW groups with pH 10 (Fig.2) AIW uptake was found to be inhibitory against ammonium production. In order to study dynamics of amino acids in large intestines, we examined free amino acids in the cecal contents to find out that cysteine level is low in AIW groups whereas isoleucine level is high in one of AIW groups with pH 10, although no significant difference was identified for other amino acids.

Bibliography
1. "Verification of Alkaline Ionized Water" by Life Water Institute, Metamor Publishing Co., 1994, p.46

*2. "Official Pharmaceutical Guidelines of Japan, Vol. IT' by Japan Public Documents Association, Hirokawa PublIshin Co., 1996

*3. "Science and Technology of Functional Water" (part) by Takashi Hayakawa, Haruffito Tsuge, edited by Water Scienll cc Institute, 1999, pp.109-116

*4. 'Tasics and Effective Use of Alkaline Ionized Water" by Takashi Hayakawa, Haruhito Tsuge, edited by Tetsuji Hc kudou, 25th General Assembly of Japan Medical Congress 'Tunctional Water in Medical Treatment", Administratio~ Offices, 1999, pp. 10- 11

Effects of alkaline ionized water on formation & maintenance of osseous tissues
by Rei Takahashi Zhenhua Zhang Yoshinori Itokawa
(Kyoto University Graduate School of Medicine, Dept. of Pathology and Tumor Biology, Fukui Prefectural University)

Effects of calcium alkaline ionized water on formation and maintenance of osseous tissues in rats were examined. In the absence of calcium in the diet, no apparent calcification was observed with only osteoid formation being prominent. Striking differences were found among groups that were given diets with 30% and 60% calcium. Rats raised by calcium ionized water showed the least osteogenetic disturbance. Tibiae and humeri are more susceptible to calcium deficiency than femora. Theses results may indicate that calcium in drinking water effectively supplements osteogenesis in case of dietary calcium deficiency. The mechanism involved in osteoid formation such as absorption rate of calcium from the intestine and effects of calcium alkaline ionized drinking water on maintaining bone structure in the process of aging or under the condition of calcium deficiency is investigated.

Osteoporosis that has lately drawn public attention is defined as "conditions of bone brittleness caused by reduction in the amount of bone frames and deterioration of osseous microstructure." Abnormal calcium metabolism has been considered to be one of the factors to contribute to this problem, which in turn is caused by insufficient calcium take in, reduction in enteral absorption rate of calcium and increase in the amount of calcium in urinal discharge. Under normal conditions, bones absorb old bones by regular metabolism through osteoid formation to maintain their strength and function as supporting structure. It is getting clear that remodeling of bones at the tissue level goes through the process of activation, resorption, reversal, matrix synthesis and mineralization. Another important function of bones is storing minerals especially by coordinating with intestines and kidneys to control calcium concentration in the blood. When something happens to this osteo metabolism, it results in abnormal morphological changes. Our analyses have been focusing mostly on the changes in the amount of bones to examine effects of calcium alkaline ionized water on the reaction system of osteo metabolism and its efficiency. Ibis time, however, we studied it further from the standpoint of histology. In other words, we conducted comparative studies on morphological and kinetic changes of osteogenesis by testing alkaline ionized water, tap water and solution of lactate on rats.
Three week old male Wistar rats were divided into 12 groups by conditions of feed and drinking water. Feeds were prepared with 0%, 30%, 60% and 100% of normal amount of calcium and were given freely. Three types of drinking water, tap water (city water, about 6ppm of Ca), calcium lactate solution (Ca=40ppm) and alkaline ionized water (Ca =40ppm, pH=9, produced by an electrolyzer NDX 4 LMC by Omco OMC Co., Ltd.) were also given keely. Rats' weight, amount of drinking water and feed as well as the content of Ca in drinking water were assayed every day. On the 19th and 25th days of testing, tetracycline hydrochloride was added to the feed for 48 hours so as to bring its concentration to 30mg/kg. On the 30th day, blood samples were taken under Nembutal anesthesia, and tibiae, humeri and femora were taken out to make non decalcified samples. Their conditions of osteoid formation and rotation were observed using Villanueva bone stain and Villanueva goldner stain.

Three groups that were given different types of drinking water and the same amount of Ca in the feed were compared to find out no significant difference in the rate of weight gain and intakes of feed and drinking water. Alkaline ionized water group had significantly greater amount of tibiae and humeri with higher concentration of calcium in the bones.

The group of 0% calcium in the feed saw drastic increase in the amount of osteoid. There was not much difference by types of drinking water. Almost no tetracycline was taken into tibiae and humeri, although a small amount was identified in ferora. As a result, osteogenesis went as far as osteoid formation, but it was likely that decalcification has not happened yet, or most of newly formed bones were absorbed.

As to the groups of 30% and 60% calcium in the feed, increase in the area of tetracycline take in was more identifiable with higher clarity in descending order of alkaline ionized water, calcium lactate solution and tap water groups. Especially in case of tap water group, irregularity among the areas of tetracycline take in was distinctive. The group of 100% calcium in the feed saw some improvements in osteogenesis in descending order of alkaline ionized water, calcium lactate solution and tap water. In any case, bone formation seemed to be in good condition at near normal level.

Alkaline ionized water was regarded to be effective for improvements of osteogenesis under the conditions of insufficient calcium in the feed. Also, the extent. of dysosteogenesis differed by the region. That is, tibiae and humeri tend to have more significant dysosteogenesis than femora.

In addition, there is a possibility that osteo metabolism varies depending on enteral absorption rate of calcium, adjustment of discharge from kidneys and functional adjustment of accessory thyroid in the presence of alkaline ionized water. We are now studying its impact on calcium concentration in the blood. We are also examining whether it is possible to deter bone deterioration by testing on fast aging mouse models.

Magnesium and calcium in drinking water and cardiovascular mortality

Excerpt from:
Scand J Work Environ Health 1991;17:91-4

Ragnar Rylander, MD, Håkan Bonevik, MD, Eva Rubenowitz, MD
Department of Environmental Hygiene, University of Göteborg, Göteborg, Sweden.

Data on the hardness of drinking water were collected from 27 municipalities in Sweden where the drinking water quality had remained unchanged for more than 20 years. Analyses were made of the levels of lead, cadmium, calcium, and magnesium. These water-quality data were compared with the age-adjusted mortality rate from ischemic heart and cerebrovascular disease for the period 1969-1978. Lead and cadmium were not present in detectable amounts except in one water sample. A statistically significant inverse relationship was present between hardness and mortality from cardiovascular disease for both sexes. Mortality caused by ischemic heart disease was inversely related to the magnesium content, particularly for the men (P<0.01). The rather small set of data supports results from previous studies suggesting that a high magnesium level in drinking water reduces the risk for death from ischemic heart disease, especially among men, although the possible importance of confounding factors needs further evaluation.
Key terms: cerebrovascular disease, ischemic heart disease, magnesium, water hardness.
Several epidemiologic investigations performed during recent decades have demonstrated an inverse relationship between water hardness and death from cardiovascular disease. The first observation was made in 1957 (1) and was subsequently elaborated upon in investigations in many other countries (2-4). A particularly relevant study was reported by Crawford et al (5), who followed the mortality rate in 11 English cities where the water hardness had changed between 1950 and 1960. Hardness had increased in five cities and decreased in six. Mortality from cardiovascular disease increased about 10% in the general population during the period of study. In the cities where hardness had decreased, mortality had increased by 20%.…

Evaluation of ionized calcium as a nutrient

Chen H, Kimura M, Zhu Z, Itokawa Y, The 11th symposium on Trace Nutrients Research, Japan Trace Nutrients Research Society, p131-138, 1994.

Summary: To clarify effect of ionized calcium water for drinking water in rats, 36 Male Wister rats weighing about 50g were randomly divided into 6 groups, and given following diet and drinking water : (1) Ca-sufficient diet, tap-water; (2) Ca-sufficient diet, tap-water;(3) Ca-sufficient diet, calcium lactate added-ionized calcium-water : (4) Ca-deficient diet, calcium lactate added-water ; (5) Ca deficient diet, calcium lactate added-water :(6) Ca-deficient diet, calcium lactate added ionized calcium-water. The diets were given by paired-feeding method 4 weeks and drinking water was ad libitum. The significant change of calcium concentration in the rats were was follows; Ca concentration of plasma, spleen, of plasma, spleen, kidney, testis and tibia in Ca deficient groups (4), (5), (6) were significantly low compared with these in Ca sufficient groups (1),(2),(3) Ca concentration in brain of groups (4),(5),(6) was low compared to these in groups (2), Ca concentration in heart and muscle of group (4) was low compared to Ca deficient groups (1),(2),(3), but these in group (5) drank Ca added-water was recovered and these in group (6) drank ionized-Ca-water was higher than these in any other groups. Ca concentration of liver in groups (4) were significantly lower than that in group (1),(3) and Ca concentration of liver in Ca deficient rats (groups (5),(6)) drank Ca-added-water were high compared to these in group (4). In 24 hours urine discharge of group (2) was high compared with groups (4), (5), (6). These results suggest that ionized Ca in drinking water may be active for intestinal absorption.

Calcium and magnesium in drinking water
and risk of death from cerebrovascular disease.
MEDLINE ABSTRACT
Author: Yang CY
Author Affiliation: School of Public Health, Kaohsiung Medical College, Taiwan, Republic of China. chunyuh*cc.kmc.edu.tw
Source: Stroke 1998 Feb; 29(2):411-4

BACKGROUND AND PURPOSE: Many studies have demonstrated a negative association between mortality from cardiovascular or cerebrovascular diseases and water hardness. This report examines whether calcium and magnesium in drinking water are protective against cerebrovascular disease.
METHODS: All eligible cerebrovascular deaths (17133 cases) of Taiwan residents from 1989 through 1993 were compared with deaths from other causes (17133 controls), and the levels of calcium and magnesium in drinking water of these residents were determined. Data on calcium and magnesium levels in drinking water throughout Taiwan were obtained from the Taiwan Water Supply Corporation. The control group consisted of people who died from other causes, and the controls were pair matched to the cases by sex, year of birth, and year of death.
RESULTS: The adjusted odds ratios (95% confidence interval) were 0.75 (0.65 to 0.85) for the group with water magnesium levels between 7.4 and 13.4 mg/L and 0.60 (0.52 to 0.70) for the group with magnesium levels of 13.5 mg/L or more. After adjustment for magnesium levels in drinking water, there was no difference between the groups with different levels of calcium.
CONCLUSIONS: The results of the present study show that there is a significant protective effect of magnesium intake from drinking water on the risk of cerebrovascular disease. This is an important finding for the Taiwan water industry and human health.

Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water.
Huang KC, Yang CC, Lee KT, Chien CT
Department of Family Medicine, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei, Taiwan.
KIDNEY INTERNATIONAL.
2003 Aug; 64(2):704-14.
BACKGROUND: Increased oxidative stress in end-stage renal disease (ESRD) patients may oxidize macromolecules and consequently lead to cardiovascular events during chronic hemodialysis. Electrolyzed reduced water (ERW) with reactive oxygen species (ROS) scavenging ability may have a potential effect on reduction of hemodialysis-induced oxidative stress in ESRD patients. METHODS: We developed a chemiluminescence emission spectrum and high-performance liquid chromatography analysis to assess the effect of ERW replacement on plasma ROS (H2O2 and HOCl) scavenging activity and oxidized lipid or protein production in ESRD patients undergoing hemodialysis. Oxidized markers, dityrosine, methylguanidine, and phosphatidylcholine hydroperoxide, and inflammatory markers, interleukin 6 (IL-6), and C-reactive protein (CRP) were determined. RESULTS: Although hemodialysis efficiently removes dityrosine and creatinine, hemodialysis increased oxidative stress, including phosphatidylcholine hydroperoxide, and methylguanidine. Hemodialysis reduced the plasma ROS scavenging activity, as shown by the augmented reference H2O2 and HOCl counts (Rh2o2 and Rhocl, respectively) and decreased antioxidative activity (expressed as total antioxidant status in this study). ERW administration diminished hemodialysis-enhanced Rh2o2 and Rhocl, minimized oxidized and inflammatory markers (CRP and IL-6), and partly restored total antioxidant status during 1-month treatment. CONCLUSION: This study demonstrates that hemodialysis with ERW administration may efficiently increase the H2O2- and HOCl-dependent antioxidant defense and reduce H2O2- and HOCl-induced oxidative stress.

Effect of Electrolytic Water (Ionized Water) Intake on Lifespan of Autoimmune Disease Prone Mice
Research from Texas University
Recent studies on electrolyzed water indicate that anode or acidic water is most effective as disinfectants; whereas, reduced or alkaline water processed through cathode is used as safe drinking water. The present drinking water study was undertaken in two strains of autoimmune disease prone mice to establish the spontaneous disease process and longevity. Weanling MRL/lpr and NZBxNZW [B/W] F1 female mice were provided daily with (1) tap water [pH ~7.5, oxygen reduction potential (ORP)~600+] (2) electrolyzed water with pH of ~9.0 and ORP ~400- and (3) hyper-reduced water with pH~10.0 and ORP~600-. Mice were provided H2O and chow diet ad libitum and weekly body weights and spontaneous deaths were recorded. The mean survival data recorded as days for MRL/lpr mice [25 mice/group] is as follows: (1) tap water 235±25, (2) reduced water 287±40 and (3) hyper-reduced water 346±45 days [<0.05]. In the case of B/W mice [25 mice/group], (1) tap water 269±16, (2) reduced water 298±19 and (3) hyper-reduced 302±18 days. A significantly decreased (<0.05) serum lipid peroxides were observed in mice fed hyper-reduced H2O. Also, the source of water did not alter lymphocyte subsets or their response to mitogens. In summary, hyper-reduced water with pH~10.0 appears to inhibit autoimmune disease of MRL/lpr mice whereas only a modest increased lifespan was noted for B/W mice. The increased lifespan by electrolyzed H2O appears to be related to the changes in free radicals and antioxidant enzyme levels. [Supported in part by Zanix Co. and Mr. Waterman Co., Tokyo, Japan].

Effect of alkaline ionized water on reproduction in gestational and lactational rats.
Watanabe T
Department of Veterinary Physiological Chemistry, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
JOURNAL OF TOXICOLOGICAL SCIENCES.
1995 May; 20(2):135-42.
Alkaline ionized water (AKW) produced by electrolysis was given to gestational and lactational rats, and its effect on dams, growth of fetuses and offsprings were investigated. The results showed that the intake of food and water in dams increased significantly when AKW was given from the latter half of the gestation period and from the former half of the lactation period. Body weight of the offsprings in the test group, both males and females, increased significantly from the latter half of the lactation period. During the lactation period and after weaning, the offsprings in the test group showed significantly hastened appearance of abdominal hair, eruption of upper incisors, opening of eyelids and other postnatal morphological developments both in males and females, as well as earlier separation of auricle and descent of testes in males compared with the control was noted. As mentioned above, it was suggested from the observations conducted that the AKW has substantial biological effects on postnatal growth, since intake of food and water and body weight of the offsprings increased and postnatal morphological development was also accelerated.

Effects of alkaline ionized water on gastric mucosal injury induced by aspirin in rats
Yuji Naito Toshikazu Yoshikawa, Tomohisa Takagi, Tsuyoshi Ishikawa, Osamu Handa, Naoyuki Matsumoto, Kiichi Matsuyama, Nobuaki Yagi, Norimasa Yoshida, Motoharu Kondo
Kyoto Prefectural University of Medicine, First Dept. of Internal Medicine), Japan.
Summary One of the objectives of this study is to determine effects of alkaline ionized water (AIW) on acute gastric mucosal injury induced by aspirin in rats. Oral doses of acidified aspirin (200 mg/kg) resulted in linear hemorrhagic erosion and increase in myeloperoxidase (MPO) activity, an index of neutrophil infiltration, in gastric mucosa. These instances of increase in total erosion and MPO activity were inhibited by administration of AIW (pH 10.5, ORP 450mV) for two weeks. Aspirin administration resulted in early increase in values of tumor necrosis factor (TNF a /cinc2 ~ ) plasma and tissue levels. The increase in TNF a /cinc2 ~ was also inhibited by administration of ArW. These results indicate that chronic administration of AIW is effective against aspirin induced gastric mucosal injury, and that its cyto protective action is associated with inhibition of neutrophil accumulation on gastric mucosa or with decline of inflammatory cytokine production. Purpose of tests Hopeful examples of clinical application of alkaline ionized water to gastrointestinal disorders are as follows: 1) Non ulcer dyspepsia (NUD) (at epigastria) 2) Irritable bowel syndrome (IBS) 3) Constipation caused by constitutional disorders (ex. diabetes, hypothyroid syndrome). 4) Peptic ulcer disease 5) Habitual users of non steroid anti inflammatory drug (NSIAD).

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ACID IONIZED WATER

Inactivation of Escherichia coli (O157:H7) and Listeria monocytogenes on plastic kitchen cutting boards by electrolyzed oxidizing water.
Venkitanarayanan KS, Ezeike GO, Hung YC, Doyle MP.
Department of Animal Science, University of Connecticut, Storrs 06269, USA.
One milliliter of culture containing a five-strain mixture of Escherichia coli O157:H7 (approximately 10(10) CFU) was inoculated on a 100-cm2 area marked on unscarred cutting boards. Following inoculation, the boards were air-dried under a laminar flow hood for 1 h, immersed in 2 liters of electrolyzed oxidizing water or sterile deionized water at 23 degrees C or 35 degrees C for 10 or 20 min; 45 degrees C for 5 or 10 min; or 55 degrees C for 5 min. After each temperature-time combination, the surviving population of the pathogen on cutting boards and in soaking water was determined. Soaking of inoculated cutting boards in electrolyzed oxidizing water reduced E. coli O157:H7 populations by > or = 5.0 log CFU/100 cm2 on cutting boards. However, immersion of cutting boards in deionized water decreased the pathogen count only by 1.0 to 1.5 log CFU/100 cm2. Treatment of cutting boards inoculated with Listeria monocytogenes in electrolyzed oxidizing water at selected temperature-time combinations (23 degrees C for 20 min, 35 degrees C for 10 min, and 45 degrees C for 10 min) substantially reduced the populations of L. monocytogenes in comparison to the counts recovered from the boards immersed in deionized water. E. coli O157:H7 and L. monocytogenes were not detected in electrolyzed oxidizing water after soaking treatment, whereas the pathogens survived in the deionized water used for soaking the cutting boards. This study revealed that immersion of kitchen cutting boards in electrolyzed oxidizing water could be used as an effective method for inactivating foodborne pathogens on smooth, plastic cutting boards.
PMID: 10456736 [PubMed - indexed for MEDLINE]

The bactericidal effects of electrolyzed oxidizing water on bacterial strains involved in hospital infections.
Vorobjeva NV, Vorobjeva LI, Khodjaev EY.
Artif Organs.
2004 Jun;28(6):590-2.

Department of Physiology of Microorganisms, Biology Faculty, Moscow State University, Lenin Hills 1/12, Moscow 119992, Russia. nvvorobjeva@mail.ru

The study is designed to investigate bactericidal actions of electrolyzed oxidizing water on hospital infections. Ten of the most common opportunistic pathogens are used for this study. Cultures are inoculated in 4.5 mL of electrolyzed oxidizing (EO) water or 4.5 mL of sterile deionized water (control), and incubated for 0, 0.5, and 5 min at room temperature. At the exposure time of 30 s the EO water completely inactivates all of the bacterial strains, with the exception of vegetative cells and spores of bacilli which need 5 min to be killed. The results indicate that electrolyzed oxidizing water may be a useful disinfectant for hospital infections, but its clinical application has still to be evaluated.
PMID: 15153153 [PubMed - in process]

Effect of electrolyzed water on wound healing.
Artif Organs.
2000 Dec;24(12):984-7.
Yahagi N, Kono M, Kitahara M, Ohmura A, Sumita O, Hashimoto T, Hori K, Ning-Juan C, Woodson P, Kubota S, Murakami A, Takamoto S.
Department of Anesthesiology, Teikyo University Mizonokuchi Hospital, Tokyo, Japan. naokiyah@aol.com
Electrolyzed water accelerated the healing of full-thickness cutaneous wounds in rats, but only anode chamber water (acid pH or neutralized) was effective. Hypochlorous acid (HOCl), also produced by electrolysis, was ineffective, suggesting that these types of electrolyzed water enhance wound healing by a mechanism unrelated to the well-known antibacterial action of HOCl. One possibility is that reactive oxygen species, shown to be electron spin resonance spectra present in anode chamber water, might trigger early wound healing through fibroblast migration and proliferation.
PMID: 11121980 [PubMed - indexed for MEDLINE]

Effect of electrolyzed oxidizing water and hydrocolloid occlusive dressings on excised burn-wounds in rats.
Chin J Traumatol.
2003 Aug 1;6(4):234-7.

Xin H, Zheng YJ, Hajime N, Han ZG.
Department of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Jilin 130031, China. xinhua7254@yahoo.com.cn

OBJECTIVE: To study the efficacy of electrolyzed oxidizing water (EOW) and hydrocolloid occlusive dressings in the acceleration of epithelialization in excised burn-wounds in rats. METHODS: Each of the anesthetized Sprague-Dawley rats (n=28) was subjected to a third-degree burn that covered approximately 10% of the total body surface area. Rats were assigned into four groups: Group I (no irrigation), Group II (irrigation with physiologic saline), Group III (irrigation with EOW) and Group IV (hydrocolloid occlusive dressing after EOW irrigation). Wounds were observed macroscopically until complete epithelialization was present, then the epithelialized wounds were examined microscopically. RESULTS: Healing of the burn wounds was the fastest in Group IV treated with hydrocolloid occlusive dressing together with EOW. Although extensive regenerative epidermis was seen in each Group, the proliferations of lymphocytes and macrophages associated with dense collagen deposition were more extensive in Group II, III and IV than in Group I. These findings were particularly evident in Group III and IV. CONCLUSIONS: Wound Healing may be accelerated by applying a hydrocolloid occlusive dressing on burn surfaces after they are cleaned with EOW.
PMID: 12857518 [PubMed - indexed for MEDLINE]

Decomposition of ethylene, a flower-senescence hormone, with electrolyzed anode water.
Biosci Biotechnol Biochem.
2003 Apr;67(4):790-6.
Harada K, Yasui K.
Department of Research and Development, Hokkaido Electric Power Co., Inc., 2-1 Tsuishikari, Ebetsu, Hokkaido 067-0033, Japan. kharada@h1.hotcn.ne.jp
Electrolyzed anode water (EAW) markedly extended the vase life of cut carnation flowers. Therefore, a flower-senescence hormone involving ethylene decomposition by EAW with potassium chloride as an electrolyte was investigated. Ethylene was added externally to EAW, and the reaction between ethylen and the available chlorine in EAW was examined. EAW had a low pH value (2.5), a high concentration of dissolved oxygen, and extremely high redox potential (19.2 mg/l and 1323 mV, respectively) when available chlorine was at a concentration of about 620 microns. The addition of ethylene to EAW led to ethylene decomposition, and an equimolar amount of ethylene chlorohydrine with available chlorine was produced. The ethylene chlorohydrine production was greatly affected by the pH value (pH 2.5, 5.0 and 10.0 were tested), and was faster in an acidic solution. Ethylene chlorohydrine was not produced after ethylene had been added to EAW at pH 2.6 when available chlorine was absent, but was produced after potassium hypochlorite had been added to such EAW. The effect of the pH value of EAW on the vase life of cut carnations was compatible with the decomposition rate of ethylene in EAW of the same pH value. These results suggest that the effect of EAW on the vase life of cut carnations was due to the decomposition of ethylene to ethylene chlorohydrine by chlorine from chlorine compounds.
PMID: 12784619 [PubMed - indexed for MEDLINE]

LIVESTOCK AND HORTICULTURE

Treatment of Escherichia coli (O157:H7) inoculated alfalfa seeds and sprouts with electrolyzed oxidizing water.
Int J Food Microbiol.
2003 Sep 15;86(3):231-7.

Department of Agricultural and Biological Engineering, Pennsylvania State University, University Park, PA 16802, USA.

Electrolyzed oxidizing water is a relatively new concept that has been utilized in agriculture, livestock management, medical sterilization, and food sanitation. Electrolyzed oxidizing (EO) water generated by passing sodium chloride solution through an EO water generator was used to treat alfalfa seeds and sprouts inoculated with a five-strain cocktail of nalidixic acid resistant Escherichia coli O157:H7. EO water had a pH of 2.6, an oxidation-reduction potential of 1150 mV and about 50 ppm free chlorine. The percentage reduction in bacterial load was determined for reaction times of 2, 4, 8, 16, 32, and 64 min. Mechanical agitation was done while treating the seeds at different time intervals to increase the effectiveness of the treatment. Since E. coli O157:H7 was released due to soaking during treatment, the initial counts on seeds and sprouts were determined by soaking the contaminated seeds/sprouts in 0.1% peptone water for a period equivalent to treatment time. The samples were then pummeled in 0.1% peptone water and spread plated on tryptic soy agar with 5 microg/ml of nalidixic acid (TSAN). Results showed that there were reductions between 38.2% and 97.1% (0.22-1.56 log(10) CFU/g) in the bacterial load of treated seeds. The reductions for sprouts were between 91.1% and 99.8% (1.05-2.72 log(10) CFU/g). An increase in treatment time increased the percentage reduction of E. coli O157:H7. However, germination of the treated seeds reduced from 92% to 49% as amperage to make EO water and soaking time increased. EO water did not cause any visible damage to the sprouts.
PMID: 12915034 [PubMed - indexed for MEDLINE]

Comparison of electrolyzed oxidizing water with various antimicrobial interventions to reduce Salmonella species on poultry.
Poult Sci.
2002 Oct;81(10):1598-605.
Fabrizio KA, Sharma RR, Demirci A, Cutter CN.
Department of Food Science, The Pennsylvania State University, University Park 16802, USA.
Foodborne pathogens in cell suspensions or attached to surfaces can be reduced by electrolyzed oxidizing (EO) water; however, the use of EO water against pathogens associated with poultry has not been explored. In this study, acidic EO water [EO-A; pH 2.6, chlorine (CL) 20 to 50 ppm, and oxidation-reduction potential (ORP) of 1,150 mV], basic EO water (EO-B; pH 11.6, ORP of -795 mV), CL, ozonated water (OZ), acetic acid (AA), or trisodium phosphate (TSP) was applied to broiler carcasses inoculated with Salmonella Typhimurium (ST) and submerged (4 C, 45 min), spray-washed (85 psi, 25 C, 15 s), or subjected to multiple interventions (EO-B spray, immersed in EO-A; AA or TSP spray, immersed in CL). Remaining bacterial populations were determined and compared at Day 0 and 7 of aerobic, refrigerated storage. At Day 0, submersion in TSP and AA reduced ST 1.41 log10, whereas EO-A water reduced ST approximately 0.86 log10. After 7 d of storage, EO-A water, OZ, TSP, and AA reduced ST, with detection only after selective enrichment. Spray-washing treatments with any of the compounds did not reduce ST at Day 0. After 7 d of storage, TSP, AA, and EO-A water reduced ST 2.17, 2.31, and 1.06 log10, respectively. ST was reduced 2.11 log10 immediately following the multiple interventions, 3.81 log10 after 7 d of storage. Although effective against ST, TSP and AA are costly and adversely affect the environment. This study demonstrates that EO water can reduce ST on poultry surfaces following extended refrigerated storage.

PMID: 12412930 [PubMed - indexed for MEDLINE]

Use of Alkali Water on Dairy Farms

Two studies follow: one in Japan, one in Pennsylvania, US.

Japanese Study

With the advent of electrolysis water treatment in the Japanese marketplace, electrolyzed alkali water was introduced into dairy farms. Knowing the positive health benefits and results that were acquired through human consumption, alkaline water was used in place of tap water as the sole source of water for dairy cows. The results are reported in the subsequent findings. The findings were obtained from 27 dairy farms, along with a report from a group of veterinarians. The source of each report is identified at the beginning of the report.
In general, the following measurable conditions were noted:
1. An increase in milk output by 18% - 28%.
2. A notable improvement in the quality of milk.
3. Elimination of strong feces and urine odors.
4. Healthier skin condition.
5. Minimized injury to the udder.
6. Decrease in diarrhea cases.
7. Strengthening of the legs.
8. Increased appetite.
9. Able to reduce mineral supplements normally added to the feed.
10. Due to an improved health condition coupled with stronger legs, thed productive life span of the cows was extended.
11. Improved the fertility rate and reduced still-births.

Aside from the above, the following observations were noted by the veterinarians:
1. A noticeably increased appetite; no new supplements were added to their diet.Increase in appetite noted in older cows as well.
2. Food well digested.
3. A beautiful sheen on the cow's hair.
4. Higher fertility rate; higher pregnancy rate.
5. New-born calves fed with alkaline water matured quicker.
6. A dramatic increase in milk production.
7. Improved liver condition.
8. Strengthened legs.
9. Minimizing of sicknesses; tremendously improved health condition. Fewer visits by vets.
10. No adverse conditions noted from consumption of alkaline water.

The following are individual findings noted by each dairy farmer who replaced tap water with alkali water.

A. Dairy Farm: Kasahara Ranch
Location: Nomura, Hokkaido
Spokesperson: Mr. G. Kasahara
1. The milk output increased from 7,000kg to 8,900kg, an increase of 27%.
2. The use of the alkali water instilled a preventive approach to the overall health condition of the dairy cow in lieu of reactive medical means. The overall health condition of the herd improved dramatically.

B. Dairy Farm: Shikawa Ranch
Location: Momembetsu, Hokkaido
Spokesperson: Mr. T. Shikawa
1. There was a noticeable improvement in the quality of the milk.
2. Despite the high temperature during the summer months, milk output had increased dramatically. During the previous ummer months, milk output had declined.

C. Dairy Farm: Sudo Ranch
Location: Munetani, Hokkaido
Spokesperson: Mr. M. Sudo
NOTE: Unlike other dairy farms, this farmer had discontinued the use of alkali water to measure the effects of returning to normal tap water. The following were the effects noted:
1. The strong odors of the excrement returned after a period of time; (the foul odor had been eliminated through the consumption of alkali water.
2. The sheen that was once present on the cows had disappeared and the hair returned to a lackluster condition.
3. The frequency of diarrhea had increased.
4. Weakness was noticed in the cows legs as opposed to the strengthening of the cows legs during the use of alkali water.

D. Dairy Farm: Takahashi Ranch
Location: Notsuke, Hokkaido
Spokesperson: Mr. Takahashi.
1. The sickness rate was considerably reduced.

E. Dairy Farm: Hamanasu Ranch
Location: Mombetsu, Hokkaido
Spokesperson: Mr. S. Nakagawa.
1. The coloring of the udder became extremely healthy.
2. Due to the alkali consumption and it's natural healing ability, the amount of injury to the udder had diminished.
3. The milk output has increased by 800 kg per cow. (NOTE: since there was no "before and after" numbers provided, the percentage increase could not be determined.)

F. Dairy Farm: Karita Ranch
Location: Notsuke, Hokkaido
Spokesperson: Mr. H. Karita

1. The results were excellent in every manner. Milk production was considerably higher, the sickness rate was down, problems associated with diarrhea were minimized, the foul odor from the excrement was gone, the cow's appetite was up, the sheen on the cow's hair was considerably higher and the overall quality of the milk was up.

G. Dairy Farm: Sunnydale Ranch
Location: Hyotsu, Hokkaido
Spokesperson: Mr. M. Danshora
1. In prior years, in an effort to increase milk production, increased feed was given to cows. With the use of alkali water, the need for increased feed was minimized.
2. Despite the pregnancy of the cow, the amount of milk production has not decreased. In prior pregnancies, the amount of milk production had decreased. This was noted in 9 out of 10 cows.
3. The improved health condition of the cows along with the stronger legs have reduced the turnover of cows. This has considerably improved the productive life span of each cow.

H. Dairy Farm: No Name Given
Location: Mombetsu, Hokkaido
Spokesperson: Mr. T. Yamaguchi
1. The overall skin condition of each cow had improved dramatically.
2. The foul odors associated with excrements and urine were eliminated with the consumption of alkali water.
3. The farm was able to reduce the amount of mineral supplements that were being added to the diet on account of the alkali water.
4. The newly born calves experienced no diarrhea.

I. Dairy Farm: Koizumi Ranch
Location: Kamikawa, Hokkaido
Spokesperson: Mr. T. Koizumi
1. The recovery period for cows giving birth had improved noticeably with the consumption of alkali water.
2. The cows have experienced increased appetite.
3. Despite the higher temperature during the ummer months, the milk output had increased dramatically.
4. The consumption of alkali water had stabilized the pH factor for each cow


J. Dairy Farm: Honami MBB Ranch
Location: Joro, Hokkaido
Spokesperson: Mr. Y. Takigawa
1. There was a remarkable improvement in the quality of milk.
2. The cows increased their water intake which resulted in increased milk production
3. The cows experienced reduced diarrhea.
4. There was a remarkable improvement in the hair and skin texture of every cow.

K. Dairy Farm: Aneshi Ranch
Location: Esachi, Hokkaido
Spokesperson: Mr. K. Aneshi
1. Due to the consumption of alkali water and the improved immunity levels, there were fewer injuries to the cow's udder during the milking process.
2. The milk output had increased from 282 tons to 360 tons or a 28% increase.
3. It was a financially and economically-wise decision to use electrolysis alkali water.

L. Dairy Farm: Royal Farm
Location: Kamikawa, Hokkaido
Spokesperson: Mr. T. Sawamoto
1. The milk output had increased from a range of 7,000 to 7,300 kg to a higher output of 9,000 kg or a 28% increase.
2. Due to the unstable water condition, the farm had gone to electrolysis water. This decision ended up being a financially-wise decision.

M. Dairy Farm: Nogyo Kyosai Dairy Association
Location: Kushiro, Hokkaido
Spokesperson: Mr. M. Sugiyama
1. The use of alkali water has considerably reduced the number of sick cows and dramatically improved the overall health condition.
2. The farm has not measured all the positive effects brought about by the alkali water but on the other hand have not experienced any negative effects.
3. One noticeable difference was their improved digestion.

N. Dairy Farm: Okura Ranch
Location: Asahi-kawa, Hokkaido
Spokesperson: Mr. Y. Okura
1. The alkali water has produced healthier cows. There were no changes to the diet or the environment but the cows became healthier.
2. Increased their monthly sales by $20,000.00 through increased milk output. (NOTE: There were no other comparative numbers provided to determine the actual increase in productivity levels.)

O. Dairy Farm: Aikawa Ranch
Location: Akan, Hokkaido
Spokesperson: Mr. M. Aikawa
1. The odors that are normally present in the urine and excrements were dramatically reduced.
2. The birthrate was considerably increased by the increase in fertility rate and the minimizing of stillborn calves.
3. There was a dramatic increase in milk production.
4. This farm is utilized as a model ranch in the use of alkali water.

P. Dairy Farm: Mitani Ranch
Location: Yubari, Hokkaido
Spokesperson: Mr. K. Mitani
1. Experienced 100% fertility and birth rates through artificial insemination.

Q. Dairy Farm: Ueda Ranch
Location: Akan, Hokkaido
Spokesperson: Mr. T. Ueda
1. The fortified calcium through the electrolysis water has strengthened the legs of the cows.
2. Due to the dramatically-improved health conditions, the quality of the milk has improved.
3. In the long run, the use of alkali water is a totally economical approach to the dairy industry.

R. Dairy Farm: Yamatani Ranch Location: Kamikawa, Hokkaido Spokesperson: Mr. M. Yamatani
1. The quality and quantity of the milk has improved considerably.
2. Considerably minimized the sickness rate of each cow.
3. Minimized diarrhea conditions.
4. An overall improvement was noted in every aspect of the dairy cow equating to better economic conditions.

S. Dairy Farm: Yamamoto Ranch Location: Amashio, Hokkaido Spokesperson: Mr.M. Yamatani
1. The milk output had increased from 317 tons to 393 tons or an increase of 24.0%
2. The cow became fertile with one month of giving birth.
3. There was a substantial reduction in the number of veterinary visits.
4. There was a noticeable increase in their appetites.

T. Dairy Farm: Saida Ranch Location: Shirahata, Hokkaido Spokesperson: Mr. K. Saida
1. The milk output had increased from 8,641 kg to 10,177kg, an increase of 17.8%

U. Dairy Farm: Fukagawa Ranch Location: Joro, Hokkaido Spokesperson: Mr. E. Fukagawa
1. There was a substantial reduction to the number of veterinary visits.
2. Reduced the swelling rate of the cow's legs.
3. Reduced the rate of external wounds caused by suction cups.

Pennsylvania Study

Increasing Dairy Milk Production With Electrolyzed Drinking Water --from "Medical News Today" --19 Mar 2008

Many different approaches are being used to increase milk production of dairy cows. A study recently completed by researchers at the University of Pennsylvania School of Veterinary Medicine (Penn Vet) indicates that improving drinking water through a technology created by EAU Technologies, has the potential to produce the desired results.

Penn Vet worked with EAU Technologies, Inc. (OTC Bulletin Board: EAUI), a leading provider of Electrolyzed Water - EMPOWERED WATER(TM) - for high- volume, business-to-business applications, for the controlled study. Dairy cows from Penn Vet's New Bolton Center campus were divided into two groups. One group's drinking water was electrolyzed, alkaline water and the control group was given regular well water. At the end of the 12-week test period, the Holstein cows showed an increase in milk production and an increase in milk fat content as well as a reduction in milk urea nitrogen (MUN.

"The electrolysis process improves the antioxidant and pH balance of the drinking water. The blood samples analyzed from the two groups indicates that the cows drinking the electrolyzed water showed differences in acid-base balance. We suspect that cows drinking the electrolyzed water had an increase in rumen activity and effectiveness; which in turn may explain the marked increased in milk butterfat," explained Dr. James Ferguson, Chief of Animal Production Systems, Department of Clinical Studies, New Bolton Center. "At the same time, the study indicated an increase in milk production for early lactating cows. The cows in the treated water group also drank more water and consumed about the same amount of feed. Bacterial Coliform levels within the EAU troughs also were significantly reduced."

The study is one of several EAU is conducting in a range of 30 to 3,000 herd dairies to measure the effectiveness of Empowered Water for milk production. As part of the Penn Vet study, in addition to the pH increase, EAU also developed a proprietary method of creating and controlling the level of measurable antioxidants in the water to better match the antioxidant conditions of a healthy cow's primary digestive system. And the EAU water also cleans the drinking water. Water samples collected from the troughs over the course of the study showed the EAU treated water was consistently negative for coliform organisms such as E. coli and other bacteria. Blood chemistry tests conducted also showed that blood urea nitrogen, creatinine, magnesium and chloride levels were lower in treated cows than those part of the control group.

"We know there are many factors that influence milk output and quality. By conducting tests in a variety of dairy settings, we believe we are gaining invaluable experience, application knowledge and acquiring accurate data to show that our Empowered Water(TM) can be an effective, natural solution, capable of impacting positive returns on milk and butterfat production. And, most importantly, benefit the overall health of the cow," added Wade Bradley, President and CEO of EAU Technologies. "This model directly mirrors EAU's business focus on providing our target industries, with high volume, robust and environmentally sound solutions."

The Penn Vet study is the first of the trial studies to be completed. Results will be published later this year. The remainder of the studies is expected to conclude over the next several months.